A Blog Around The Clock

The ‘Other’ Neglected Diseases in Global Public Health: Surgical Conditions in Sub-Saharan Africa:

Currently in sub-Saharan Africa, most patients with surgical problems that are routinely treatable in high-income countries never reach a health facility, or are treated at a facility with inadequate human or physical resources. These conditions lead to premature death or physical disability with a significant economic burden. Meanwhile, the last decade has seen the emergence of numerous “neglected tropical disease” (NTD) initiatives in global public health. As surgeons working with clinicians in sub-Saharan Africa, the momentum for NTDs causes us to ask: Shouldn’t surgical conditions also be considered “neglected”?
This article compares NTDs and surgical conditions in sub-Saharan Africa, considering their estimated burden and the cost-effectiveness of treatment, the scope of these conditions and associated global health disparities, and the effect of donor priorities on provision of surgical services. Lessons from NTD initiatives are analyzed among possible solutions to improving access to surgical services in sub-Saharan Africa.

The Ticket to Transport:

Cell nuclei are like gated communities–quite selective about who gets in. And understandably so, because if the wrong proteins showed up at the wrong time and place, the consequences could be disastrous. The standard procedure for moving large molecules that cannot diffuse from the cytoplasm into the nucleus is to use the transport proteins known as karyopherins as escorts. How do karyopherins know whether their cargo is a protein that ought to get in? By their ability to bind with recognition sites on the cargo: no recognition, no passage. The best known example is the canonical or classical nuclear localization signal (cNLS)–a specific sequence that, when added to a protein, drives its nuclear localization. But more recently, a new class of NLSs has been identified, known as a proline-tyrosine nuclear localization signal (PY-NLS). This new signal is recognized specifically by the highly conserved transporter molecule Karyopherinβ2.

Feasibility Of Preventing Malaria Parasite From Becoming Sexually Mature Demonstrated:

Researchers have demonstrated the possibility of preventing the human malaria parasite, Plasmodium falciparum, which is responsible for more than a million malaria deaths a year, from becoming sexually mature.

New Barn Swallow Study Reveals Image Makes The Bird:

In the world of birds, where fancy can be as fleeting as flight, the color of the bird apparently has a profound effect on more than just its image. A new study of barn swallows reveals it also affects the bird’s physiology.

Toad Research Could Leapfrog To New Muscle Model:

A toad sits at a pond’s edge eyeing a cricket on a blade of grass. In the blink of an eye, the toad snares the insect with its tongue. This deceptively simple, remarkably fast feeding action offers a new look at how muscles work.

Kew Gardens Provides Climate For Agricultural Change:

A device to help some of the most impoverished farmers in Africa maximise their crop yields is being tested at London’s Kew Gardens.

Factors That Make Bacteria More Modular Detailed:

Many bacteria break their metabolic processes into chunks. That may be logically tidy, but it’s often metabolically inefficient. Researchers have now figured out the factors that tend to make bacteria more modular.

A Great Lakes Mystery: The Case Of The Disappearing Species:

Throughout the overlooked depths of Lake Michigan and other Great Lakes, a small but important animal is rapidly disappearing.

Living Fossils Have Long- And Short-term Memory Despite Lacking Brain Structures Of Modern Cephalopods:

Robyn Crook from the City University of New York reports that Nautilus, the ancient living ancestors of modern cephalopods, have both long and short-term memory, despite lacking the brain structures that modern cephalopods evolved for long-term memory.

Slide Rule Sense: Amazonian Indigenous Culture Demonstrates Universal Mapping Of Number Onto Space:

The ability to map numbers onto a line, a foundation of all mathematics, is universal, says a study published May 30 in the journal Science, but the form of this universal mapping is not linear but logarithmic. The findings illuminate both the nature and the limits of the human predisposition to measurement, a foundation for science, engineering, and much of our modern culture.

Scientists Discover Stinging Truths About Jellyfish Blooms In The Bering Sea:

A new study helps explain a cyclic increase and decrease of jellyfish populations, which transformed parts of the Bering Sea–one of the U.S.’s most productive fisheries–into veritable jellytoriums during the 1990s.

How People Influence Connectivity Among Ecosystems:

Ecosystems are constantly exchanging materials through the movement of air in the atmosphere, the flow of water in rivers and the migration of animals across the landscape. People, however, have also established themselves as another major driver of connectivity among ecosystems.

Taxonomists Describe The Top 10 Most Surprising Species Discovered In 2007:

Each year the scientific community identifies around 17,000 new animals and plants. To attract people’s attention on the discovery of species, a key for their evolution, survival and conservation, an international committee of experts has just published a list of the 10 most curious and surprising species described in 2007.

How To Construct A ‘Firefly’ Worm:

Research describing a new modified luminescent worm allows, for the first time, one to measure, in real time, the metabolism of an entire living organism. The key behind this capacity relies in the fact that the luminescence is produced using the animal’s available energy, which reflects its metabolism that, as such, can be extrapolated from measuring the emitted light.

Healthy Parents Provide Clues To Survival Of Young Haddock On Georges Bank:

In 2003, haddock on Georges Bank experienced the largest baby boom ever documented for the stock, with an estimated 800 million new young fish entering the population. With typical annual averages of 50 to 100 million new fish in the last few decades, fisheries biologists have been puzzled by the huge increase and its ramifications for stock management. They have been looking for answers and may have found one – healthy adults.

Why Are Computational Neuroscience and Systems Biology So Separate?:

Despite similar computational approaches, there is surprisingly little interaction between the computational neuroscience and the systems biology research communities. In this review I reconstruct the history of the two disciplines and show that this may explain why they grew up apart. The separation is a pity, as both fields can learn quite a bit from each other. Several examples are given, covering sociological, software technical, and methodological aspects. Systems biology is a better organized community which is very effective at sharing resources, while computational neuroscience has more experience in multiscale modeling and the analysis of information processing by biological systems. Finally, I speculate about how the relationship between the two fields may evolve in the near future.

Hmmm, perhaps in some areas of biology, but I saw a great exception (there are two other exceptions noted in the first comment on the paper as well).
Ten Simple Rules for Aspiring Scientists in a Low-Income Country:

Being a scientist entails a common set of characteristics. Admiring nature and having concern for social issues; possessing a strong academic background, team work abilities, honesty, discipline, skepticism, communication skills, competitiveness, ability to accept and give criticism, and productive relationships are some of the most obvious traits that scientists should have. To be a scientist in a low-income country (LIC), however, requires a complementary set of qualities that are necessary to confront the drawbacks that work against the development of science. The failure of many young researchers to mature as professional scientists upon their return to their country from advanced training elsewhere, motivated us to propose these ten rules.

This is something I’ll forwards to my scientist friends in Belgrade….

U.S. Reporters Often Do A Poor Job Of Reporting About New Medical Treatments, Analysis Finds:

Most medical news stories about health interventions fail to adequately address costs, harms, benefits, the quality of evidence, and the existence of other treatment options, finds a new analysis in this week’s PLoS Medicine. The analysis was conducted by Gary Schwitzer from the University of Minnesota School of Journalism and Mass Communication.

Sedentary High School Girls Are At Significant Risk For Future Osteoporosis:

Significant numbers of female high school athletes and non-athletes suffer from one or more components of the female athlete triad, a combination of three conditions that can lead to cardiovascular disease, according to a new study by Medical College of Wisconsin researchers in Milwaukee.

Tourists To Caribbean Urged To Pay One Dollar Each To Help Fight Tropical Diseases Of Poverty:

Away from the beaches, resorts, and cruise ships of the Caribbean, there lies a hidden underbelly of poverty and with this poverty comes endemic neglected tropical diseases (NTDs). In an editorial in this month’s PLoS Neglected Tropical Diseases, the journal’s Editor in Chief, Professor Peter Hotez (George Washington University and Sabin Vaccine Institute) proposes that a modest US $1.00 airline or cruise ship tax or a tax on tourist entry could provide a funding mechanism for the Caribbean countries to control these NTDs.

First ‘Smell Map’ Supports Idea That Universal Laws For Smell Are Hard-Wired In Brain:

Is the smell of almonds closer to that of roses or bananas? Weizmann Institute scientists have now answered that question (roses) by showing for the first time that smells can be mapped and the relative distance between various odors determined. Their findings, which appeared recently in Nature Methods, may help scientists to unravel the basic laws underlying our sense of smell, as well as potentially enabling odors to be digitized and transferred via computer in the future.

How Fairness Is Wired In The Brain:

In the biblical story in which two women bring a baby to King Solomon, both claiming to be the mother, he suggests dividing the child so that each woman can have half. Solomon’s proposed solution, meant to reveal the real mother, also illustrates an issue central to economics and moral philosophy: how to distribute goods fairly.

Did Walking On Two Feet Begin With A Shuffle?:

Somewhere in the murky past, between four and seven million years ago, a hungry common ancestor of today’s primates, including humans, did something novel. While temporarily standing on its rear feet to reach a piece of fruit, this protohominid spotted another juicy morsel in a nearby shrub and began shuffling toward it instead of dropping on all fours, crawling to the shrub and standing again.

World’s Rarest Rhinos Make First Video Trap Appearance — Then Toss Camera:

After just a month in operation, specially designed video cameras installed to capture wildlife footage in the jungles of South East Asia have twice recorded remarkable images of a mother and child pair of the world’s rarest rhino.

Altruism In Social Insects Is A Family Affair:

The contentious debate about why insects evolved to put the interests of the colony over the individual has been reignited by new research from the University of Leeds, showing that they do so to increase the chances that their genes will be passed on.

Heat, Not Light, May Be Real Engine Driving Biodiversity:

What causes tropical life to thrive: temperature, or sunlight? The answer is not necessarily “both.” According to a study recently published online in PNAS Early Edition, the explosion of species at the tropics has much more to do with warmth than with light.

Saltwater Sleuths: Seeking Clues To Help Determine Ages Of Fish And Shellfish Populations:

Fishery biologist Sandy Sutherland looks through the lens of the microscope at tiny sections of fish earbones, known as otoliths, each showing annual bands of growth. She carefully counts the bands to determine the age of the fish, then moves on to the next sample. Known as an age reader, Sutherland is one of a small team at NOAA’s Northeast Fisheries Science Center (NEFSC) whose aging work is critical to stock assessments needed to manage the nation’s fishery resources in the Northwest Atlantic Ocean.

Common Aquatic Animal’s Genome Can Capture Foreign DNA:

Long viewed as straitlaced spinsters, sexless freshwater invertebrate animals known as bdelloid rotifers may actually be far more promiscuous than anyone had imagined: Scientists at Harvard University have found that the genomes of these common creatures are chock-full of DNA from plants, fungi, bacteria, and animals.

Mind Over Matter: Monkey Feeds Itself Using Its Brain:

A monkey has successfully fed itself with fluid, well-controlled movements of a human-like robotic arm by using only signals from its brain, researchers from the University of Pittsburgh School of Medicine report in the journal Nature. This significant advance could benefit development of prosthetics for people with spinal cord injuries and those with “locked-in” conditions such as Lou Gehrig’s disease, or amyotrophic lateral sclerosis.

Authentic Viking DNA Retrieved From 1,000-year-old Skeletons:

Although “Viking” literally means “pirate,” recent research has indicated that the Vikings were also traders to the fishmongers of Europe. Stereotypically, these Norsemen are usually pictured wearing a horned helmet but in a new study, Jørgen Dissing and colleagues from the University of Copenhagen, investigated what went under the helmet; the scientists were able to extract authentic DNA from ancient Viking skeletons, avoiding many of the problems of contamination faced by past researchers.

Giant Flying Reptiles Preferred To Walk:

New research into gigantic flying reptiles has found that they weren’t all gull-like predators grabbing fish from the water but that some were strongly adapted for life on the ground.

Childhood Lead Exposure Is Associated With Decreased Brain Volume In Adults:

Childhood exposure to lead is associated with shrinking (“volume loss”) of specific parts of the brain in adulthood, finds a related study in this week’s PLoS Medicine. Dr Kim Cecil and colleagues (University of Cincinnati, USA) studied the association between exposure to lead in the uterus and during early childhood and brain volume in adulthood.

Battling Bird Flu By The Numbers:

A pair of Los Alamos National Laboratory theorists have developed a mathematical tool that could help health experts and crisis managers determine in real time whether an emerging infectious disease such as avian influenza H5N1 is poised to spread globally.

Large-scale Community Protein Annotation — WikiProteins:

Today sees the launch of a new collaborative website initially focusing on proteins and their role in biology and medicine. The WikiProfessional technology underlying the site has been developed based upon the collaborative Wikipedia approach. WikiProteins provides a method for community annotation on a huge scale.

Climate Change Already Affecting U.S. Water, Land, And Biodiversity, Report Finds:

The U.S. Climate Change Science Program (CCSP) has released “Synthesis and Assessment Product 4.3 (SAP 4.3): The Effects of Climate Change on Agriculture, Land Resources, Water Resources, and Biodiversity in the United States.” The CCSP integrates the federal research efforts of 13 agencies on climate and global change. This report is one of the most extensive examinations of climate impacts on U.S. ecosystems.

First Female DNA Sequenced:

Geneticists of Leiden University Medical Centre (LUMC) are the first to determine the DNA sequence of a woman. She is also the first European whose DNA sequence has been determined. Following in-depth analysis, the sequence will be made public, except incidental privacy-sensitive findings. The results will contribute to insights into human genetic diversity.

Why Are Some People Unable To Express Their Emotions?:

Italian investigators have published a new study on the neurobiologic correlates of the inability to express emotions (alexithymia) in the third 2008 issue of Psychotherapy and Psychosomatics.

DNA Clues To Reproductive Behavior:

A species of wild yeast goes through a cycle of sexual reproduction once in every 1,000 asexual generations, according to new research by Imperial biologists published in the PNAS journal in April.

The Secret Behind Silkworm’s Hardy Stomachs:

Silkworms have a unique ability to eat toxic mulberry leaves without feeling ill, and researchers have come one step closer to understanding why: silkworms contain a special digestive enzyme that is not affected by mulberry’s toxic chemicals.

Scientists Announce Top 10 New Species In Last Year:

The International Institute for Species Exploration at Arizona State University and an international committee of taxonomists — scientists responsible for species exploration and classification — has just announced the top 10 new species described in 2007.

More here and here.
Fundamental Building Block In Flowering Plants Evolved Independently, Yet Almost Identically In Ancient Plants:

Biologists have discovered that a fundamental building block in the cells of flowering plants evolved independently, yet almost identically, on a separate branch of the evolutionary tree–in an ancient plant group called lycophytes that originated at least 420 million years ago.

New Research Forces U-turn In Population Migration Theory:

Research led by the University of Leeds has discovered genetic evidence that overturns existing theories about human migration into Island Southeast Asia (covering the Philippines, Indonesia and Malaysian Borneo) – taking the timeline back by nearly 10,000 years.

New Statistical Method Reveals Surprises About Our Ancestry:

A statistical approach to studying genetic variation promises to shed new light on the history of human migration.

There are 48 articles published this week in PLoS ONE, something for everyone. Read, note, comment, blog about and send trackbacks… Here are some of the titles I found cool:
Evidence of Authentic DNA from Danish Viking Age Skeletons Untouched by Humans for 1,000 Years:

Given the relative abundance of modern human DNA and the inherent impossibility for incontestable proof of authenticity, results obtained on ancient human DNA have often been questioned. The widely accepted rules regarding ancient DNA work mainly affect laboratory procedures, however, pre-laboratory contamination occurring during excavation and archaeological-/anthropological handling of human remains as well as rapid degradation of authentic DNA after excavation are major obstacles. We avoided some of these obstacles by analyzing DNA from ten Viking Age subjects that at the time of sampling were untouched by humans for 1,000 years. We removed teeth from the subjects prior to handling by archaeologists and anthropologists using protective equipment. An additional tooth was removed after standard archaeological and anthropological handling. All pre-PCR work was carried out in a “clean- laboratory” dedicated solely to ancient DNA work. Mitochondrial DNA was extracted and overlapping fragments spanning the HVR-1 region as well as diagnostic sites in the coding region were PCR amplified, cloned and sequenced. Consistent results were obtained with the “unhandled” teeth and there was no indication of contamination, while the latter was the case with half of the “handled” teeth. The results allowed the unequivocal assignment of a specific haplotype to each of the subjects, all haplotypes being compatible in their character states with a phylogenetic tree drawn from present day European populations. Several of the haplotypes are either infrequent or have not been observed in modern Scandinavians. The observation of haplogroup I in the present study (<2% in modern Scandinavians) supports our previous findings of a pronounced frequency of this haplogroup in Viking and Iron Age Danes. The present work provides further evidence that retrieval of ancient human DNA is a possible task provided adequate precautions are taken and well-considered sampling is applied.

A Reappraisal of Azhdarchid Pterosaur Functional Morphology and Paleoecology:

Azhdarchid pterosaurs were among the most widespread and successful of pterosaur clades, but their paleoecology remains controversial. Morphological features common to all azhdarchids include a long, shallow rostrum; elongate, cylindrical cervical vertebrae that formed a long and unusually inflexible neck; and proportionally short wings with an abbreviated fourth phalanx. While azhdarchids have been imagined as vulture-like scavengers, sediment probers, swimmers, waders, aerial predators, or stork-like generalists, most recent authors have regarded them as skim-feeders, trawling their lower jaws through water during flight and seizing aquatic prey from the water’s surface. Although apparently widely accepted, the skim-feeding model lacks critical support from anatomy and functional morphology. Azhdarchids lack the many cranial specialisations exhibited by extant skim-feeding birds, most notably the laterally compressed lower jaw and shock absorbing apparatus required for this feeding style. Well-preserved azhdarchid skulls are rare, but their rostra and lower jaws appear to have been sub-triangular in cross-section, and thus dissimilar to those of skim-feeders and sediment probers. Taphonomic data indicates that azhdarchids predominately inhabited inland settings, and azhdarchid morphology indicates that they were poorly suited for all proposed lifestyles bar wading and terrestrial foraging. However, azhdarchid footprints show that their feet were relatively small, padded and slender, and thus not well suited for wading. We argue that azhdarchids were stork- or ground hornbill-like generalists, foraging in diverse environments for small animals and carrion. Proficient terrestrial abilities and a relatively inflexible neck are in agreement with this interpretation.

Function of the Shaw Potassium Channel within the Drosophila Circadian Clock:

In addition to the molecular feedback loops, electrical activity has been shown to be important for the function of networks of clock neurons in generating rhythmic behavior. Most studies have used over-expression of foreign channels or pharmacological manipulations that alter membrane excitability. In order to determine the cellular mechanisms that regulate resting membrane potential (RMP) in the native clock of Drosophila we modulated the function of Shaw, a widely expressed neuronal potassium (K+) channel known to regulate RMP in Drosophila central neurons. We show that Shaw is endogenously expressed in clock neurons. Differential use of clock gene promoters was employed to express a range of transgenes that either increase or decrease Shaw function in different clusters of clock neurons. Under LD conditions, increasing Shaw levels in all clock neurons (LNv, LNd, DN1, DN2 and DN3), or in subsets of clock neurons (LNd and DNs or DNs alone) increases locomotor activity at night. In free-running conditions these manipulations result in arrhythmic locomotor activity without disruption of the molecular clock. Reducing Shaw in the DN alone caused a dramatic lengthening of the behavioral period. Changing Shaw levels in all clock neurons also disrupts the rhythmic accumulation and levels of Pigment Dispersing Factor (PDF) in the dorsal projections of LNv neurons. However, changing Shaw levels solely in LNv neurons had little effect on locomotor activity or rhythmic accumulation of PDF. Based on our results it is likely that Shaw modulates pacemaker and output neuronal electrical activity that controls circadian locomotor behavior by affecting rhythmic release of PDF. The results support an important role of the DN clock neurons in Shaw-mediated control of circadian behavior. In conclusion, we have demonstrated a central role of Shaw for coordinated and rhythmic output from clock neurons.

Blood Parasites in Owls with Conservation Implications for the Spotted Owl (Strix occidentalis):

The three subspecies of Spotted Owl (Northern, Strix occidentalis caurina; California, S. o. occidentalis; and Mexican, S. o. lucida) are all threatened by habitat loss and range expansion of the Barred Owl (S. varia). An unaddressed threat is whether Barred Owls could be a source of novel strains of disease such as avian malaria (Plasmodium spp.) or other blood parasites potentially harmful for Spotted Owls. Although Barred Owls commonly harbor Plasmodium infections, these parasites have not been documented in the Spotted Owl. We screened 111 Spotted Owls, 44 Barred Owls, and 387 owls of nine other species for haemosporidian parasites (Leucocytozoon, Plasmodium, and Haemoproteus spp.). California Spotted Owls had the greatest number of simultaneous multi-species infections (44%). Additionally, sequencing results revealed that the Northern and California Spotted Owl subspecies together had the highest number of Leucocytozoon parasite lineages (n = 17) and unique lineages (n = 12). This high level of sequence diversity is significant because only one Leucocytozoon species (L. danilewskyi) has been accepted as valid among all owls, suggesting that L. danilewskyi is a cryptic species. Furthermore, a Plasmodium parasite was documented in a Northern Spotted Owl for the first time. West Coast Barred Owls had a lower prevalence of infection (15%) when compared to sympatric Spotted Owls (S. o. caurina 52%, S. o. occidentalis 79%) and Barred Owls from the historic range (61%). Consequently, Barred Owls on the West Coast may have a competitive advantage over the potentially immune compromised Spotted Owls.

iTools: A Framework for Classification, Categorization and Integration of Computational Biology Resources:

The advancement of the computational biology field hinges on progress in three fundamental directions – the development of new computational algorithms, the availability of informatics resource management infrastructures and the capability of tools to interoperate and synergize. There is an explosion in algorithms and tools for computational biology, which makes it difficult for biologists to find, compare and integrate such resources. We describe a new infrastructure, iTools, for managing the query, traversal and comparison of diverse computational biology resources. Specifically, iTools stores information about three types of resources-data, software tools and web-services. The iTools design, implementation and resource meta – data content reflect the broad research, computational, applied and scientific expertise available at the seven National Centers for Biomedical Computing. iTools provides a system for classification, categorization and integration of different computational biology resources across space-and-time scales, biomedical problems, computational infrastructures and mathematical foundations. A large number of resources are already iTools-accessible to the community and this infrastructure is rapidly growing. iTools includes human and machine interfaces to its resource meta-data repository. Investigators or computer programs may utilize these interfaces to search, compare, expand, revise and mine meta-data descriptions of existent computational biology resources. We propose two ways to browse and display the iTools dynamic collection of resources. The first one is based on an ontology of computational biology resources, and the second one is derived from hyperbolic projections of manifolds or complex structures onto planar discs. iTools is an open source project both in terms of the source code development as well as its meta-data content. iTools employs a decentralized, portable, scalable and lightweight framework for long-term resource management. We demonstrate several applications of iTools as a framework for integrated bioinformatics. iTools and the complete details about its specifications, usage and interfaces are available at the iTools web page http://iTools.ccb.ucla.edu.

General Practice and Pandemic Influenza: A Framework for Planning and Comparison of Plans in Five Countries:

Although primary health care, and in particular, general practice will be at the frontline in the response to pandemic influenza, there are no frameworks to guide systematic planning for this task or to appraise available plans for their relevance to general practice. We aimed to develop a framework that will facilitate planning for general practice, and used it to appraise pandemic plans from Australia, England, USA, New Zealand and Canada. We adapted the Haddon matrix to develop the framework, populating its cells through a multi-method study that incorporated the peer-reviewed and grey literature, interviews with general practitioners, practice nurses and senior decision-makers, and desktop simulation exercises. We used the framework to analyse 89 publicly-available jurisdictional plans at similar managerial levels in the five countries. The framework identifies four functional domains: clinical care for influenza and other needs, public health responsibilities, the internal environment and the macro-environment of general practice. No plan addressed all four domains. Most plans either ignored or were sketchy about non-influenza clinical needs, and about the contribution of general practice to public health beyond surveillance. Collaborations between general practices were addressed in few plans, and inter-relationships with the broader health system, even less frequently. This is the first study to provide a framework to guide general practice planning for pandemic influenza. The framework helped identify critical shortcomings in available plans. Engaging general practice effectively in planning is challenging, particularly where governance structures for primary health care are weak. We identify implications for practice and for research.

The Dark Side of the Salad: Salmonella typhimurium Overcomes the Innate Immune Response of Arabidopsis thaliana and Shows an Endopathogenic Lifestyle:

Salmonella enterica serovar typhimurium contaminated vegetables and fruits are considerable sources of human infections. Bacteria present in raw plant-derived nutrients cause salmonellosis, the world wide most spread food poisoning. This facultative endopathogen enters and replicates in host cells and actively suppresses host immune responses. Although Salmonella survives on plants, the underlying bacterial infection mechanisms are only poorly understood. In this report we investigated the possibility to use Arabidopsis thaliana as a genetically tractable host system to study Salmonella-plant interactions. Using green fluorescent protein (GFP) marked bacteria, we show here that Salmonella can infect various Arabidopsis tissues and proliferate in intracelullar cellular compartments. Salmonella infection of Arabidopsis cells can occur via intact shoot or root tissues resulting in wilting, chlorosis and eventually death of the infected organs. Arabidopsis reacts to Salmonella by inducing the activation of mitogen-activated protein kinase (MAPK) cascades and enhanced expression of pathogenesis related (PR) genes. The induction of defense responses fails in plants that are compromised in ethylene or jasmonic acid signaling or in the MKK3-MPK6 MAPK pathway. These findings demonstrate that Arabidopsis represents a true host system for Salmonella, offering unique possibilities to study the interaction of this human pathogen with plants at the molecular level for developing novel drug targets and addressing current safety issues in human nutrition.

Prolonged Visual Experience in Adulthood Modulates Holistic Face Perception:

Using the well-known composite illusion as a marker of the holistic perception of faces, we tested how prolonged visual experience with a specific population of faces (4- to 6-year-old children) modulates the face perception system in adulthood. We report a face composite effect that is larger for adult than children faces in a group of adults without experience with children faces (“children-face novices”), while it is of equal magnitude for adults and children faces in a population of preschool teachers (“children-face experts”). When considering preschool teachers only, we observed a significant correlation between the number of years of experience with children faces and the differential face composite effect between children and adults faces. Participants with at least 10 years of qualitative experience with children faces had a larger composite face effect for children than adult faces. Overall, these observations indicate that even in adulthood face processes can be reshaped qualitatively, presumably to facilitate efficient processing of the differential morphological features of the frequently encountered population of faces.

Cryptic Contamination and Phylogenetic Nonsense:

Ancient human DNA has been treated cautiously ever since the problems related to this type of material were exposed in the early 1990s, but as sequential genetic data from ancient specimens have been key components in several evolutionary and ecological studies, interest in ancient human DNA is on the increase again. It is especially tempting to approach archaeological and anthropological questions through this type of material, but DNA from ancient human tissue is notoriously complicated to work with due to the risk of contamination with modern human DNA. Various ways of authenticating results based on ancient human DNA have been developed to circumvent the problems. One commonly used method is to predict what the contamination is expected to look like and then test whether the ancient human DNA fulfils this prediction. If it does, the results are rejected as contamination, while if it does not, they are often considered authentic. We show here that human contamination in ancient material may well deviate from local allele frequencies or the distributions to be found among the laboratory workers and archaeologists. We conclude that it is not reliable to authenticate ancient human DNA solely by showing that it is different from what would be expected from people who have handled the material.

Brain Networks for Integrative Rhythm Formation:

Performance of externally paced rhythmic movements requires brain and behavioral integration of sensory stimuli with motor commands. The underlying brain mechanisms to elaborate beat-synchronized rhythm and polyrhythms that musicians readily perform may differ. Given known roles in perceiving time and repetitive movements, we hypothesized that basal ganglia and cerebellar structures would have greater activation for polyrhythms than for on-the-beat rhythms. Using functional MRI methods, we investigated brain networks for performing rhythmic movements paced by auditory cues. Musically trained participants performed rhythmic movements at 2 and 3 Hz either at a 1:1 on-the-beat or with a 3:2 or a 2:3 stimulus-movement structure. Due to their prior musical experience, participants performed the 3:2 or 2:3 rhythmic movements automatically. Both the isorhythmic 1:1 and the polyrhythmic 3:2 or 2:3 movements yielded the expected activation in contralateral primary motor cortex and related motor areas and ipsilateral cerebellum. Direct comparison of functional MRI signals obtained during 3:2 or 2:3 and on-the-beat rhythms indicated activation differences bilaterally in the supplementary motor area, ipsilaterally in the supramarginal gyrus and caudate-putamen and contralaterally in the cerebellum. The activated brain areas suggest the existence of an interconnected brain network specific for complex sensory-motor rhythmic integration that might have specificity for elaboration of musical abilities.

Microbiological Implications of Periurban Agriculture and Water Reuse in Mexico City:

Recycled treated or untreated wastewater represents an important health challenge in developing countries due to potential water related microbiological exposure. Our aim was to assess water quality and health implications in a Mexico City periurban agricultural area. A longitudinal study in the Xochimilco wetland area was conducted, and 42 sites were randomly selected from 211, including irrigation water canals and effluents of treatment plants. Sample collection took place during rainy and dry seasons (2000-2001). Microbiological parameters (total coliforms, fecal coliforms, streptococci/enterococci, and bacteria other than Vibrio grown on TCBS), Helicobacter pylori, and physicochemical parameters including trihalomethanes (THM) were determined. Fecal coliforms and fecal streptococci are appropriate indicators of human or animal fecal contamination. Fecal coliform counts surpass Mexican and World Health Organization irrigation water guidelines. Identified microorganisms associated with various pathologies in humans and domestic animals comprise Escherichia coli, Klebsiella spp., Salmonella spp., Enterobacter spp., Enterococcus spp., and Pseudomonas spp; H. pylori was also present in the water. An environmental characteristic of the canal system showed high Total Organic Carbon content and relatively low dissolved oxygen concentration; residual chlorine as a disinfection control is not efficient, but THMs do not represent a problem. During the rainy season, temperature and conductivity were higher; in contrast, pH, dissolved oxygen, ammonia, and residual chlorine were lower. This is related with the continuous load of feces from human and animal sources, and to the aquatic systems, which vary seasonally and exhibit evidence of lower water quality in effluents from treatment plants. There is a need for improvement of wastewater treatment systems, as well as more efficient monitoring, regulation, and enforcement procedures for wastewater disposal into bodies of water.

Why Are Clinicians Not Embracing the Results from Pivotal Clinical Trials in Severe Sepsis? A Bayesian Analysis:

Five pivotal clinical trials (Intensive Insulin Therapy; Recombinant Human Activated Protein C [rhAPC]; Low-Tidal Volume; Low-Dose Steroid; Early Goal-Directed Therapy [EGDT]) demonstrated mortality reduction in patients with severe sepsis and expert guidelines have recommended them to clinical practice. Yet, the adoption of these therapies remains low among clinicians. We selected these five trials and asked: Question 1-What is the current probability that the new therapy is not better than the standard of care in my patient with severe sepsis? Question 2-What is the current probability of reducing the relative risk of death (RRR) of my patient with severe sepsis by meaningful clinical thresholds (RRR >15%; >20%; >25%)? Bayesian methodologies were applied to this study. Odds ratio (OR) was considered for Question 1, and RRR was used for Question 2. We constructed prior distributions (enthusiastic; mild, moderate, and severe skeptic) based on various effective sample sizes of other relevant clinical trials (unfavorable evidence). Posterior distributions were calculated by combining the prior distributions and the data from pivotal trials (favorable evidence). Answer 1-The analysis based on mild skeptic prior shows beneficial results with the Intensive Insulin, rhAPC, and Low-Tidal Volume trials, but not with the Low-Dose Steroid and EGDT trials. All trials’ results become unacceptable by the analyses using moderate or severe skeptic priors. Answer 2-If we aim for a RRR>15%, the mild skeptic analysis shows that the current probability of reducing death by this clinical threshold is 88% for the Intensive Insulin, 62-65% for the Low-Tidal Volume, rhAPC, EGDT trials, and 17% for the Low-Dose Steroid trial. The moderate and severe skeptic analyses show no clinically meaningful reduction in the risk of death for all trials. If we aim for a RRR >20% or >25%, all probabilities of benefits become lower independent of the degree of skepticism. Our clinical threshold analysis offers a new bedside tool to be directly applied to the care of patients with severe sepsis. Our results demonstrate that the strength of evidence (statistical and clinical) is weak for all trials, particularly for the Low-Dose Steroid and EGDT trials. It is essential to replicate the results of each of these five clinical trials in confirmatory studies if we want to provide patient care based on scientifically sound evidence.

A Critical Assessment of the Effects of Bt Transgenic Plants on Parasitoids:

The ecological safety of transgenic insecticidal plants expressing crystal proteins (Cry toxins) from the bacterium Bacillus thuringiensis (Bt) continues to be debated. Much of the debate has focused on nontarget organisms, especially predators and parasitoids that help control populations of pest insects in many crops. Although many studies have been conducted on predators, few reports have examined parasitoids but some of them have reported negative impacts. None of the previous reports were able to clearly characterize the cause of the negative impact. In order to provide a critical assessment, we used a novel paradigm consisting of a strain of the insect pest, Plutella xylostella (herbivore), resistant to Cry1C and allowed it to feed on Bt plants and then become parasitized by Diadegma insulare, an important endoparasitoid of P. xylostella. Our results indicated that the parasitoid was exposed to a biologically active form of the Cy1C protein while in the host but was not harmed by such exposure. Parallel studies conducted with several commonly used insecticides indicated they significantly reduced parasitism rates on strains of P. xylostella resistant to these insecticides. These results provide the first clear evidence of the lack of hazard to a parasitoid by a Bt plant, compared to traditional insecticides, and describe a test to rigorously evaluate the risks Bt plants pose to predators and parasitoids.

Environmental Factors Contributing to the Spread of H5N1 Avian Influenza in Mainland China:

Since late 2003, highly pathogenic avian influenza (HPAI) outbreaks caused by infection with H5N1 virus has led to the deaths of millions of poultry and more than 10 thousands of wild birds, and as of 18-March 2008, at least 373 laboratory-confirmed human infections with 236 fatalities, have occurred. The unrestrained worldwide spread of this disease has caused great anxiety about the potential of another global pandemic. However, the effect of environmental factors influencing the spread of HPAI H5N1 virus is unclear. A database including incident dates and locations was developed for 128 confirmed HPAI H5N1 outbreaks in poultry and wild birds, as well as 21 human cases in mainland China during 2004-2006. These data, together with information on wild bird migration, poultry densities, and environmental variables (water bodies, wetlands, transportation routes, main cities, precipitation and elevation), were integrated into a Geographical Information System (GIS). A case-control design was used to identify the environmental factors associated with the incidence of the disease. Multivariate logistic regression analysis indicated that minimal distance to the nearest national highway, annual precipitation and the interaction between minimal distance to the nearest lake and wetland, were important predictive environmental variables for the risk of HPAI. A risk map was constructed based on these factors. Our study indicates that environmental factors contribute to the spread of the disease. The risk map can be used to target countermeasures to stop further spread of the HPAI H5N1 at its source.

Orientation Sensitivity at Different Stages of Object Processing: Evidence from Repetition Priming and Naming:

An ongoing debate in the object recognition literature centers on whether the shape representations used in recognition are coded in an orientation-dependent or orientation-invariant manner. In this study, we asked whether the nature of the object representation (orientation-dependent vs orientation-invariant) depends on the information-processing stages tapped by the task. We employed a repetition priming paradigm in which briefly presented masked objects (primes) were followed by an upright target object which had to be named as rapidly as possible. The primes were presented for variable durations (ranging from 16 to 350 ms) and in various image-plane orientations (from 0° to 180°, in 30° steps). Significant priming was obtained for prime durations above 70 ms, but not for prime durations of 16 ms and 47 ms, and did not vary as a function of prime orientation. In contrast, naming the same objects that served as primes resulted in orientation-dependent reaction time costs. These results suggest that initial processing of object identity is mediated by orientation-independent information and that orientation costs in performance arise when objects are consolidated in visual short-term memory in order to be reported.

How Do US Journalists Cover Treatments, Tests, Products, and Procedures? An Evaluation of 500 Stories:

* The daily delivery of news stories about new treatments, tests, products, and procedures may have a profound–and perhaps harmful–impact on health care consumers.
* A US Web site project, HealthNewsReview.org (http://HealthNewsReview.org/), modeled after similar efforts in Australia and Canada, evaluates and grades health news coverage, notifying journalists of their grades.
* After almost two years and 500 stories, the project has found that journalists usually fail to discuss costs, the quality of the evidence, the existence of alternative options, and the absolute magnitude of potential benefits and harms.
* Reporters and writers have been receptive to the feedback; editors and managers must be reached if change is to occur.
* Time (to research stories), space (in publications and broadcasts), and training of journalists can provide solutions to many of the journalistic shortcomings identified by the project.

Disease Mongering Is Now Part of the Global Health Debate:

Disease mongering is the selling of sickness that widens the boundaries of illness in order to grow markets for those who sell and deliver treatments. It is a process that turns healthy people into patients, causes iatrogenic harm, and wastes precious resources [1]. Disease mongering is the contemporary form of “medicalisation.” It is a process now driven by both corporate and professional interests, and it has become part of the global debate about health care. International consumer groups now target drug company-backed disease mongering as a wasteful threat to public health [2], while the global pharmaceutical industry has been forced to defend its promotion of “lifestyle” medicines for problems like slimming and sexual difficulties [3].

False Hopes, Unwarranted Fears: The Trouble with Medical News Stories:

On April 26, 2007, ABC World News, the American Broadcasting Corporation’s flagship television news program, aired a “good news” story about a new test for prostate cancer [1]. Against a background of a dramatic graphic showing that 1.6 million American men undergo prostate biopsy each year, the presenter announced: “Researchers at Johns Hopkins say they have developed a more accurate blood screening test.” The story was based on a new study examining the performance of early prostate cancer antigen-2 as a serum marker for prostate cancer [2]. Unfortunately, ABC failed to disclose one crucial fact: the principal investigator of this study receives a share of the royalty sales of the test and is a paid consultant to the test’s manufacturer [3].

Sperm Sociality: Cooperation, Altruism, and Spite:

A swimming sperm cell appears to perfectly capture the individualist Darwinian struggle, as it frantically races onwards towards a waiting egg. Consistent with this imagery, sperm morphology and behaviour in many organisms appears exquisitely designed to maximise the chances of fertilisation of each individual sperm cell [1]. However, there are numerous less obliging cases where sperm seem poorly suited to the task, even to the extent that the majority of sperm in an ejaculate may be infertile [2,3]. Why would such sperm evolve?

Sniffing Out the Right Address:

Olfactory systems in organisms ranging from invertebrates to mammals distinguish between odors using an array of olfactory neurons that express different olfactory receptors–usually just one receptor in a given neuron. Since an organism’s genome can encode hundreds of olfactory receptors, researchers have struggled to understand how a single olfactory neuron decides which receptor to express. In Drosophila, it seems that regulatory elements upstream of olfactory receptor genes act like zip codes to direct the expression of each gene to the appropriate class of olfactory neurons.

When Skin Damage Causes Death:

Our skin routinely shields us from microbes, allergens, and other environmental assaults, a yeoman’s service we often take for granted–until that barrier is breached. In response to injury, be it a simple cut or a deep wound, keratinocytes, the cells that form the epidermal layer, proliferate and dispatch chemical messengers to enlist the healing services of immune cells. But new research shows that sometimes damaged skin can send the wrong message to its immune cell partners. Rather than recruiting immune cells to repair a wound, Raphael Kopan and colleagues report, defective skin can trigger a systemic, ultimately fatal immune response.

Climate Change Does Double-whammy To Animals In Seasonal Environments:

Plant-eating animals in highly seasonal environments, such as the Arctic, are struggling to locate nutritious food as a result of climate change, according to research that will be published in the 21 May 2008 online edition of the journal Proceedings of the Royal Society B. Led by Penn State Associate Professor of Biology Eric Post, the research, which focused on caribou, suggests that not only are these animals arriving at their breeding grounds too late in the season to enjoy the peak availability of food–the focus of previous research by Post–but they also are suffering from a reduced ability to locate the few high-quality plants that remain before these plants, too, become unavailable.

Related….
Tidal Cycle Could Amplify Global-warming Related Sea-level Rises:

The results of several scientific studies conducted since 1993 have confirmed a 3.2 cm sea level rise. Although this variation might appear negligible, it has in fact turned out to be twice as high as that recorded over the whole of the previous century. This increase in sea level is a consequence of global warming. When sea temperature rises, the sea expands and therefore occupies a greater volume. This phenomenon is now well known to scientists, but other processes that have received less research attention, such as the tidal cycle, seem to contribute at global scale just as much to changes in sea level.

What Is The Value Of Biodiversity To Our Collective Future?:

What will the loss of biodiversity cost us in the long term? How much do national economies need to invest now in order to stop the trend? And what price will we have to pay if we do not act? These are the questions the TEEB – The Economics of Eco-systems and Biodiversity – project is seeking to answer.

Honey Bee Losses Continue To Rise In U.S.:

Colony Collapse Disorder, diseases, parasitic mites and other stressors continue to take a devastating toll on U.S. honey bee populations, but Pennsylvania beekeepers on average fared better than their counterparts nationally during this past winter, according to apiculture experts in Penn State’s College of Agricultural Sciences.

Male-Male Courtship Pattern Shaped By Emergence Of A New Gene In Fruit Flies:

When a young gene known as sphinx is inactivated in the common fruit fly, it leads to increased male-male courtship, scientists report in the May 27, 2008, issue of the Proceedings of the National Academy of Sciences. High levels of male-male courtship are widespread in many fly species, but not in Drosophila melanogaster, the tiny insect that has been a mainstay of genetic research for more than a century.

Fixing The Education Digital Disconnect One Video Game At A Time: FAS Launches Immune Attack:

The Federation of American Scientists (FAS) has just launched Immune Attack TM, an exciting, fun and fast-moving video game that teaches the critical scientific facts of immunology.